other:
Recombinant:Article Title: A protocol to use Drosophila melanogaster larvae to model human glioblastoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Repo monoclonal, primary antibody, to label glial cell nuclei, final dilution 1:10 Developmental Studies Hybridoma Bank Cat #8D12 Alexa Fluor 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L), final dilution 1:400 Jackson Immunolabs Cat #715-586-151 Alexa Fluor 647 AffiniPure Goat AntiHorseradish Peroxidase, to label neuronal cell types, final dilution 1:100 Jackson Immunolabs Cat #123-605-021 Chemicals, peptides, and recombinant proteins 103 Phosphate-buffered saline (PBS) pH 7.4 Cellgro Cat #46-013-CM 32% paraformaldehyde Electron Microscopy Sciences Cat #15714 Triton X-100 Santa Cruz Biotechnology Cat #sc-29112 1% sodium azide G-Biosciences Cat #786-299 Normal goat serum Cell Signaling Technology Cat #5425 Vectashield Antifade Mounting Medium Vector Laboratories Cat #H-1000 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat #H-1200 Experimental models: Organisms/strains UAS-dEGFRl on X chromosome Drosophila stock (Read et al., 2009) Dr. Gertrud Schupbach (schupbac@ princeton.edu) On request UAS-dp110CAAX on X chromosome Drosophila stock (Read et al., 2009) Bloomington Drosophila Stock Center Stock# 8294 (Continued on next page) 4 STAR Protocols 3, 101609, September 16, 2022 ..
Saline:Article Title: A protocol to use Drosophila melanogaster larvae to model human glioblastoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Repo monoclonal, primary antibody, to label glial cell nuclei, final dilution 1:10 Developmental Studies Hybridoma Bank Cat #8D12 Alexa Fluor 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L), final dilution 1:400 Jackson Immunolabs Cat #715-586-151 Alexa Fluor 647 AffiniPure Goat AntiHorseradish Peroxidase, to label neuronal cell types, final dilution 1:100 Jackson Immunolabs Cat #123-605-021 Chemicals, peptides, and recombinant proteins 103 Phosphate-buffered saline (PBS) pH 7.4 Cellgro Cat #46-013-CM 32% paraformaldehyde Electron Microscopy Sciences Cat #15714 Triton X-100 Santa Cruz Biotechnology Cat #sc-29112 1% sodium azide G-Biosciences Cat #786-299 Normal goat serum Cell Signaling Technology Cat #5425 Vectashield Antifade Mounting Medium Vector Laboratories Cat #H-1000 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat #H-1200 Experimental models: Organisms/strains UAS-dEGFRl on X chromosome Drosophila stock (Read et al., 2009) Dr. Gertrud Schupbach (schupbac@ princeton.edu) On request UAS-dp110CAAX on X chromosome Drosophila stock (Read et al., 2009) Bloomington Drosophila Stock Center Stock# 8294 (Continued on next page) 4 STAR Protocols 3, 101609, September 16, 2022 ..
Electron Microscopy:Article Title: A protocol to use Drosophila melanogaster larvae to model human glioblastoma.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Repo monoclonal, primary antibody, to label glial cell nuclei, final dilution 1:10 Developmental Studies Hybridoma Bank Cat #8D12 Alexa Fluor 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H+L), final dilution 1:400 Jackson Immunolabs Cat #715-586-151 Alexa Fluor 647 AffiniPure Goat AntiHorseradish Peroxidase, to label neuronal cell types, final dilution 1:100 Jackson Immunolabs Cat #123-605-021 Chemicals, peptides, and recombinant proteins 103 Phosphate-buffered saline (PBS) pH 7.4 Cellgro Cat #46-013-CM 32% paraformaldehyde Electron Microscopy Sciences Cat #15714 Triton X-100 Santa Cruz Biotechnology Cat #sc-29112 1% sodium azide G-Biosciences Cat #786-299 Normal goat serum Cell Signaling Technology Cat #5425 Vectashield Antifade Mounting Medium Vector Laboratories Cat #H-1000 Vectashield Antifade Mounting Medium with DAPI Vector Laboratories Cat #H-1200 Experimental models: Organisms/strains UAS-dEGFRl on X chromosome Drosophila stock (Read et al., 2009) Dr. Gertrud Schupbach (schupbac@ princeton.edu) On request UAS-dp110CAAX on X chromosome Drosophila stock (Read et al., 2009) Bloomington Drosophila Stock Center Stock# 8294 (Continued on next page) 4 STAR Protocols 3, 101609, September 16, 2022 ..
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